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Addgene inc pcxle hul
Pcxle Hul, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcxle-hul/pCXLE-hUL+(Plasmid+%2327080)/pmc13001064-58-15-16
Average 96 stars, based on 363 article reviews
pcxle hul - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.
Article Snippet: Briefly, 53105 hUC-MSCs were electroporated with 3mg of each episomal plasmid, including pCXLEhSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900mV, 500ms and 1 pulse.

Article Title: Selection method of iPS cell, preparation method of iPS cell, and control device
Article Snippet: FIG. 10 is the drawing illustrating the composition of the pCXLE-hOct3/4-SfRE1 vector in Example. (1) pCXLE-hUL The vector pCXLE-hUL was purchased from Addgene (see FIG. 8). (2) pCXLE-hSK-Oki_mut1 The vector pCXLE-hSK-Oki_mut1 was prepared such that the Oki_mut1 luciferase may express as the luminescent reporter protein together with Sox2 and Klf4.

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
Article Snippet: Briefly, 5×10 5 hUC-MSCs were electroporated with 3μg of each episomal plasmid, including pCXLE-hSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900μV, 500ms and 1 pulse.

Article Title: Generation of an induced pluripotent stem cell line SDUBMSi005-A from a patient with double primary gastric and colon carcinoma.
Article Snippet: After 7 days, PBMCs were electroporated with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLE-hSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene) using the Human CD34+ Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza).

Article Title: Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism
Article Snippet: Skin fibroblasts were reprogrammed by nucleofection with pCXLE-hOct3/4 (RRID:Addgene_27076), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) plasmids using the Amaxa nucleofection kit for human dermal fibroblasts (Lonza, VPD-100) and program P-022 of the Nucleofector 2b (Lonza).

Article Title: Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1.
Article Snippet: Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCXLE-hUL (Addgene #27080), pCXLE-hSK (Addgene #27078), and pCXLE-hOct3/4 (Addgene #27076)

Article Title: Establishment of patient-specific induced pluripotent stem cell line SDUBMSi009-A from a patient with X-linked Lowe syndrome.
Article Snippet: The Human CD34 + Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza) were used to nucleofection with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLEhSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene).

Article Title: Novel Source for Tooth Regeneration: Human induced Pluripotent Stem Cells derived Odontogenic Mesenchymal Stem Cells
Article Snippet: Plasmid sequences and information were obtained from Addgene: pCXLE-hOCT3/4-shp53-F (RRID:Addgene_27077), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) (10).

Luciferase:

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.
Article Snippet: Briefly, 53105 hUC-MSCs were electroporated with 3mg of each episomal plasmid, including pCXLEhSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900mV, 500ms and 1 pulse.

Article Title: Selection method of iPS cell, preparation method of iPS cell, and control device
Article Snippet: FIG. 10 is the drawing illustrating the composition of the pCXLE-hOct3/4-SfRE1 vector in Example. (1) pCXLE-hUL The vector pCXLE-hUL was purchased from Addgene (see FIG. 8). (2) pCXLE-hSK-Oki_mut1 The vector pCXLE-hSK-Oki_mut1 was prepared such that the Oki_mut1 luciferase may express as the luminescent reporter protein together with Sox2 and Klf4.

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
Article Snippet: Briefly, 5×10 5 hUC-MSCs were electroporated with 3μg of each episomal plasmid, including pCXLE-hSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900μV, 500ms and 1 pulse.

Article Title: Generation of an induced pluripotent stem cell line SDUBMSi005-A from a patient with double primary gastric and colon carcinoma.
Article Snippet: After 7 days, PBMCs were electroporated with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLE-hSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene) using the Human CD34+ Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza).

Article Title: Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism
Article Snippet: Skin fibroblasts were reprogrammed by nucleofection with pCXLE-hOct3/4 (RRID:Addgene_27076), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) plasmids using the Amaxa nucleofection kit for human dermal fibroblasts (Lonza, VPD-100) and program P-022 of the Nucleofector 2b (Lonza).

Article Title: Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1.
Article Snippet: Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCXLE-hUL (Addgene #27080), pCXLE-hSK (Addgene #27078), and pCXLE-hOct3/4 (Addgene #27076)

Article Title: Establishment of patient-specific induced pluripotent stem cell line SDUBMSi009-A from a patient with X-linked Lowe syndrome.
Article Snippet: The Human CD34 + Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza) were used to nucleofection with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLEhSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene).

Article Title: Novel Source for Tooth Regeneration: Human induced Pluripotent Stem Cells derived Odontogenic Mesenchymal Stem Cells
Article Snippet: Plasmid sequences and information were obtained from Addgene: pCXLE-hOCT3/4-shp53-F (RRID:Addgene_27077), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) (10).

Expressing:

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.
Article Snippet: Briefly, 53105 hUC-MSCs were electroporated with 3mg of each episomal plasmid, including pCXLEhSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900mV, 500ms and 1 pulse.

Article Title: Selection method of iPS cell, preparation method of iPS cell, and control device
Article Snippet: FIG. 10 is the drawing illustrating the composition of the pCXLE-hOct3/4-SfRE1 vector in Example. (1) pCXLE-hUL The vector pCXLE-hUL was purchased from Addgene (see FIG. 8). (2) pCXLE-hSK-Oki_mut1 The vector pCXLE-hSK-Oki_mut1 was prepared such that the Oki_mut1 luciferase may express as the luminescent reporter protein together with Sox2 and Klf4.

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
Article Snippet: Briefly, 5×10 5 hUC-MSCs were electroporated with 3μg of each episomal plasmid, including pCXLE-hSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900μV, 500ms and 1 pulse.

Article Title: Generation of an induced pluripotent stem cell line SDUBMSi005-A from a patient with double primary gastric and colon carcinoma.
Article Snippet: After 7 days, PBMCs were electroporated with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLE-hSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene) using the Human CD34+ Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza).

Article Title: Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism
Article Snippet: Skin fibroblasts were reprogrammed by nucleofection with pCXLE-hOct3/4 (RRID:Addgene_27076), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) plasmids using the Amaxa nucleofection kit for human dermal fibroblasts (Lonza, VPD-100) and program P-022 of the Nucleofector 2b (Lonza).

Article Title: Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1.
Article Snippet: Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCXLE-hUL (Addgene #27080), pCXLE-hSK (Addgene #27078), and pCXLE-hOct3/4 (Addgene #27076)

Article Title: Establishment of patient-specific induced pluripotent stem cell line SDUBMSi009-A from a patient with X-linked Lowe syndrome.
Article Snippet: The Human CD34 + Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza) were used to nucleofection with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLEhSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene).

Article Title: Novel Source for Tooth Regeneration: Human induced Pluripotent Stem Cells derived Odontogenic Mesenchymal Stem Cells
Article Snippet: Plasmid sequences and information were obtained from Addgene: pCXLE-hOCT3/4-shp53-F (RRID:Addgene_27077), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) (10).

shRNA:

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration.
Article Snippet: Briefly, 53105 hUC-MSCs were electroporated with 3mg of each episomal plasmid, including pCXLEhSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900mV, 500ms and 1 pulse.

Article Title: Selection method of iPS cell, preparation method of iPS cell, and control device
Article Snippet: FIG. 10 is the drawing illustrating the composition of the pCXLE-hOct3/4-SfRE1 vector in Example. (1) pCXLE-hUL The vector pCXLE-hUL was purchased from Addgene (see FIG. 8). (2) pCXLE-hSK-Oki_mut1 The vector pCXLE-hSK-Oki_mut1 was prepared such that the Oki_mut1 luciferase may express as the luminescent reporter protein together with Sox2 and Klf4.

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration
Article Snippet: Briefly, 5×10 5 hUC-MSCs were electroporated with 3μg of each episomal plasmid, including pCXLE-hSK, pCXLE-hUL and pCXLE-hOCT3/4-shp53-F (expressing SOX2, KLF4, L-MYC, LIN28, OCT3/4 and p53-targeting shRNA, Addgene, Cambridge, MA) using the following conditions: 900μV, 500ms and 1 pulse.

Article Title: Generation of an induced pluripotent stem cell line SDUBMSi005-A from a patient with double primary gastric and colon carcinoma.
Article Snippet: After 7 days, PBMCs were electroporated with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLE-hSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene) using the Human CD34+ Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza).

Article Title: Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism
Article Snippet: Skin fibroblasts were reprogrammed by nucleofection with pCXLE-hOct3/4 (RRID:Addgene_27076), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) plasmids using the Amaxa nucleofection kit for human dermal fibroblasts (Lonza, VPD-100) and program P-022 of the Nucleofector 2b (Lonza).

Article Title: Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson's disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1.
Article Snippet: Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable) pCXLE-hUL (Addgene #27080), pCXLE-hSK (Addgene #27078), and pCXLE-hOct3/4 (Addgene #27076)

Article Title: Establishment of patient-specific induced pluripotent stem cell line SDUBMSi009-A from a patient with X-linked Lowe syndrome.
Article Snippet: The Human CD34 + Cells Nucleofector Kit (Lonza) and the U-009 program on a 2B Nucleofector System (Lonza) were used to nucleofection with 2 μg of pCXLE-hOCT3/4-shp53-F, 2 μg of pCXLEhSK, 2 μg of pCXLE-hUL and 2 μg pCXWB-EBNA1 (Addgene).

Article Title: Novel Source for Tooth Regeneration: Human induced Pluripotent Stem Cells derived Odontogenic Mesenchymal Stem Cells
Article Snippet: Plasmid sequences and information were obtained from Addgene: pCXLE-hOCT3/4-shp53-F (RRID:Addgene_27077), pCXLE-hSK (RRID:Addgene_27078), and pCXLE-hUL (RRID:Addgene_27080) (10).



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